The antibody has a proven maximum biotin-streptavidin/HRP staining at a 1/5,000 - 1/10,000 dilution in rat striatum, cortex, and hippocampus. The antiserum has been characterized as specific to calbindin D-28k; please see reference listed below. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended.

Photo Description: IHC image of neurons staining for the calbindin in the rat amygdala (above) and the rat cortex (below). The tissue was fixed with 4% formaldehyde in 0.1 M phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:10000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen. The section was then mounted on slides with permount.   

Host: Rabbit

Quantity / Volume: 100 µL

State: Lyophilized Whole Serum

Reacts With: Mouse, Rat

Availability: In Stock

Alternate Names:  Calbindin; nCAB27; Calbindin D28; D-28K; Vitamin D-dependent calcium-binding protein; Vitamin D-dependent calcium-binding protein, avian-type; CALB1; calbindin 1, 28kDa, anti-Calbindin

RRID:  AB_572222Immunogen: Bovine Calbindin D-28KGene Symbol: CALB1Database Links:   Entrez Gene: 12307  Mouse  Entrez Gene: 83839  RatNCBI Gene Aliases: MGC13895