The antibody has a provenmaximum biotin-avidin/HRP staining at a 1/1000 - 1/4000 dilution in rat cortex, hippocampus and hypothalamus. The antiserum has been characterized as specific to calretinin; please see references listed below. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended

Photo Description:IHC image of neurons staining for calretinin in the rat cortex (above) and the rat dentate gyrus (below). The tissue was fixed with 4% formaldehyde in 0.1 M phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:4000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.

Host:Rabbit

Quantity / Volume: 100 µL

State: Lyophilized Whole Serum

Reacts With: Rat

Availability: In Stock

RRID: AB_572223Immunogen: Chick Calretinin fusion proteinGene Symbol: CALB2Database Links:Entrez Gene: 117059 Rat