The antibody has a proven strong Biotin-Streptavidin/HRP immunostaining at a 1/500-1/1000 dilution in human pancreatic islets. Staining is completely eliminated by pretreatment of the diluted antibody in an excess of glucagon. Preadsorption of the diluted antibody with an excess of the following substances had no effect on glucagon labeling: secretin, vasoactive intestinal peptide, peptide histidine isoleucine-27, gastric inhibitory polypeptide, rat and human growth hormone releasing hormone and somatostatin.

Photo Description: IHC image of alpha cells in the islets of Langerhans of the rat pancreas staining for glucagon (above) and counterstained with hemotoxylin (below). The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for frozen sectioning. Sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:1000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.

Host:Rabbit

Quantity / Volume: 100 µL

State: Lyophilized Whole SerumReacts With: Chick, Human, Mouse, Pig, Rat, Sea Lamprey, Snail, Three-Spined Stickleback (Fish), Turtle

Availability: In StockAlternate Names: anti-glucagon

Gene Symbol: GCGRRID: AB_572241Database Links:Entrez Gene:396196 ChickEntrez Gene:2641 HumanEntrez Gene:14526MouseEntrez Gene:397595PigEntrez Gene:24952RatEntrez Gene:102943761 Turtle