The antibody produces a strong postive labeling of LHRH / GnRH at dilution of 1/2,000 - 1,4,000 using biotin-streptavidin/HRP in rat hypothalamus (median eminence). Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended. The above image is of LHRH (GnRH) terminals in rat median eminence, ryostat sections, ABC / DAB detection.Staining is completely eliminated by pretreatment of the diluted antibody with 5 µg of LHRH per mL of diluted antiserum.
Photo Description: IHC image of staining for LNRH/GnRH in the rat median eminence. The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:4000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.
Host: Rabbit
Quantity / Volume: 100 µL
State: Lyophilized Whole Serum
Reacts With: Bat, Carp, Catfish, Chick, Chicken, Clarias Batrachus (Catfish), Fish, Frog, Frog (Green Treefrog), Gerbil, Guinea Pig, Guinea Pig, Hagfish, Hamster, Human, Lizard, Mole Rat, Monkey, Mouse, Mudpuppy (Necturus Maculosus), Octopus, Pig, Rat, Salamander, Snail, Snail (Bulla Gouldiana), Squid, Trout
Availability: In Stock
Alternate Names: Gonadotropin-Releasing Hormone; Gonadorelin; Luliberin I; Gonadoliberin I; progonadoliberin-1; GRH, anti-LHRH
Gene Symbol: GNRH1 RRID: AB_572248Database Links:
Entrez Gene:103295055 Bat Entrez Gene: 770134 Chick Entrez Gene: 100135026 Frog Entrez Gene: 100135531 Guinea Pig Entrez Gene: 100774729 Hamster Entrez Gene: 2796 Human Entrez Gene: 101705387 Mole Rat Entrez Gene: 613033 Monkey Entrez Gene: 14714 Mouse Entrez Gene: 397516 Pig Entrez Gene: 25194 Rat Entrez Gene: 100135917 Trout
