The antibody has a proven strong Biotin-Streptavidin/HRP staining at a 1/1000-1/2000 dilution in rat hypothalamus (median eminence). The specificity of the antiserum was examined by soluble pre-adsorption with the peptides in question at a final concentration of 106M. Somatostatin immunolabeling was completely abolished by pre-adsorption with somatostatin, somatostatin 25, and somatostatin 28. Pre-adsorption with the following peptides resulted in no reduction of immunostaining: substance P, amylin, glucagon, insulin, neuropeptide Y, and VIP.

Photo Description: IHC image of the median eminence staining for somatostatin (above). The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen. Florescent secondary (below).

Host: Rabbit

Quantity / Volume:  100 µL

State: Lyophilized Whole Serum

Reacts With: Alligator, Anuran (Frog), Bat, Bird, Buffalo (Bubalus Bubalis), Bullfrog, Cat, Chick, Chicken, Dermophis Mexicanus Mexican Caecilian  (Amphibian ), Dog, Duck, Fish, Fowl, Frog (Xenopus Laevis), Guinea Pig, Hamster, Human, Lizard, Malayan Pangolin (Sunda Pangolin), Manducta Sexta (Moth), Monkey, Mouse, Pig, Pigeon, Quail, Rabbit, Raccoon, Rana Pipiens (Leopard Frog), Rat, Scorpaena Porcus (Fish), Sea Bass (Fish), Sheep, Snail, Snake, Sparus Aurata (Fish), Starfish, Sting Ray, Turtle, Zebrafish

Availability: In Stock

Preabsorption Control: Available at Sigma Aldrich, Catalog # S9129

Alternate Names:  Growth hormone release-inhibiting factor; Somatostatin 14; Somatostatin 28; prepro-somatostatin; SMST; anti-somatostatin

Gene Symbol:  SSTRRID:  AB_572264Database Links:  Entrez Gene: 102569049     Alligator  Entrez Gene: 102242181     Bat  Entrez Gene: 100219295     Bird  Entrez Gene: 102394725     Buffalo  Entrez Gene: 101096911     Cat  Entrez Gene: 396279           Chicken  Entrez Gene: 403993           Dog  Entrez Gene: 101800536      Duck  Entrez Gene: 100037904      Frog  Entrez Gene: 100730851      Guinea Pig  Entrez Gene: 100774243      Hamster  Entrez Gene: 6750               Human  Entrez Gene: 708626           Monkey  Entrez Gene: 20604             Mouse  Entrez Gene: 494469           Pig  Entrez Gene: 102092386      Pigeon  Entrez Gene: 107318299      Quail  Entrez Gene: 100353697      Rabbit  Entrez Gene: 24797             Rat  Entrez Gene: 443006           Sheep  Entrez Gene: 102451183      Turtle  Entrez Gene: 79186             Zebrafish