The ImmunoStar monoclonal Tyrosine Hydroxylase antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat catecholamine neuron systems using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/4000 - 1/8000 with the biotin/avidin-HRP technique.  This antibody has been used successfully for IHC, ICC, FC, and IP.Western Blot:  This antibody does not cross react with dihydropterdine reductase, dopamine-B-hydroxylase, phenylethanolamine-N-methyltransferase, phenylalanine hydroxylase or tryptophan hydroxylase using Western Blot methods. Click here for more Western Blot protocol information.

The TH antibody identifies dopaminergic axons and nerve ending during early mouse, rat and human development. This is because tyrosine hydroxylase is expressed very early in the developing dopamine neuron phenotype.

Photo Description: High magnification (above) and low magnification (below) IHC image of the rat midbrain staining for tyrosine hydroxylase. The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% rabbit serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, rabbit anti-mouse secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen. 

Host: Mouse

Quantity / Volume: 100 µL

State: Lyophilized Whole Serum

Reacts With: Alpaca (Llama), Amphibian, Anuran (Frog), Anuran (Urodele), Aplysia Californica (Sea Slug), Bee, Bird, Blowfly, Boar, Budgerigars, Canary, Cat, Chick, Chicken, Clam, Cockroach, Cod (Fish), Crab, Crayfish, Dog, Drosophila (Fly), Eel, Emu, Enegal Bichirs (Eel), Ewe, Ferret, Fish, Fly, Fox, Frog (Xenopus Laevis), Fruit Fly, Gastropod (Slug), Gastropoda, Gecko, Gerbil, Goldfish, Guinea Pig, Guppies, Hamster (Mesocricetus Auratus), Helisoma Duryi (Snail), Hen, Human, Iguana, Ilyanassa Obsoleta (Sea Snail), Lamprey, Leech, Lizard, Lobster, Locust, Mexican Axolotl (Salamander), Mollusca, Monkey (Macaca Fascicularis), Mouse, Newt, Opossum, Parrot, Periplaneta Americana (Cockroach), Phestilla Sibogae (Sea Coral), Phestilla Sibogae (Sea Slug), Phoronis Pallida (Phoronida), Pig, Pigeon, Possum, Prawn, Quail, Rabbit, Rat, Renilla Koellikeri (Sea Pansy), Rodent, Salamander, Salmon, Sea Fish, Sea Slug, Shark, Sheep, Short-Beaked Echidna, Shrew, Snail, Snake, Sparrow, Spisula (Clam), Squirrel Monkey, Starling, Stickleback, Tadpole, Trout, Turkey, Turtle, Urodele Amphibian, Water Buffalo, Worm, Yeast, Zebra Finch, Zebrafish

Availability: In Stock

Alternate Names:  Tyrosine 3-monooxygenase (TH); TYH; Tyrosine 3-hydroxylase, anti-TH, anti-tyrosine hydroxylase

Gene Symbol:  ThDatabase Links:  Entrez Gene: 408930          Bee  Entrez Gene: 449568          Boar  Entrez Gene: 101090720    Cat  Entrez Gene: 395592         Chicken   Entrez Gene: 403444         Dog  Entrez Gene: 101682802    Ferret  Entrez Gene: 38746           Fly  Entrez Gene: 105299526    Fox  Entrez Gene: 100488900    Frog  Entrez Gene: 100734150    Guinea Pig  Entrez Gene: 100760127    Hamster  Entrez Gene: 7054             Human  Entrez Gene: 103238895    Monkey  Entrez Gene: 21823           Mouse  Entrez Gene: 102092212    Pigeon  Entrez Gene: 107314555    Quail  Entrez Gene: 100009362    Rabbit  Entrez Gene: 25085           Rat  Entrez Gene: 103186002    Shark  Entrez Gene: 101120619    Sheep  Entrez Gene: 102072945    Sparrow  Entrez Gene: 723977         Turkey  Entrez Gene: 101937054    Turtle  Entrez Gene: 100270767    Worm  Entrez Gene: 100219119    Zebra Finch  Entrez Gene: 30384           Zebrafish