The antibody has a proven strong biotin-streptavidin/HRP staining at a 1/2000 - 1/4000 dilution in rat hypothalamus. Staining is completely eliminated by pretreatment of the diluted antibody with 10 µg/mL of arginine vasopressin. Pre-adsorption with as much as 100 µg/mL of oxytocin had no effect on immunolabeling.
Photo Description: IHC image of rat hypothalamus staining for vasopressin. The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.
Host: Rabbit
Quantity / Volume: 100 µL
State: Lyophilized Whole Serum
Reacts With: Anole, Bluehead Wrasses (Fish), Chicken, Clarias Batrachus (Catfish), Cockroach, Ewe / Sheep, Hamster, Human, Hydra Vulgaris (Polyp), Locust, Mole Rat, Monkey, Mouse, Pigeon, Rat, Shrew, Squirrel
Availability: In Stock
Alternate Names: Vasopressin (Arg); Antidiuretic Hormone; Arginine vasopressin; Vasopressin-neurophysin 2-copeptin; ARG-vasopressin; prepro-AVP-NPII; neurohypophyseal; vasopressin-neurophysin II-copeptin; prepro-arginine-vasopressin-neurophysin II; AVP-NPII; VP; ADH; ARVP; AVRP, anti-vasopressin
Gene Symbol: AVPRRID: AB_572219Database Links: Entrez Gene: 396101 Chicken Entrez Gene: 443477 Ewe / Sheep Entrez Gene: 551 Human Entrez Gene: 101717720 Mole Rat Entrez Gene: 717101 Monkey Entrez Gene: 11998 Mouse Entrez Gene: 24221 Rat Entrez Gene: 102870182 Shrew