The antibody produces strong labeling of VAChT at a dilution of 1/3,000 - 1/5,000 using biotin-streptavidin/HRP technique in rat basal forebrain. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended. Immunolabeling is completely abolished by pre-adsorption with synthetic rat VAChT (511-530). Immunolabeling of transfected cells demonstrates no cross reactivity with vesicular monoamine transporters.

Photo Description: IHC image of neurons in the rat basal forebrain staining for VAT. The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% rabbit serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, rabbit anti-goat secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.

Host:Goat

Quantity / Volume: 100 µL

State: Lyophilized Whole Serum

Species Reactivity: Human, Mouse, Rat

Availability: In Stock

Alternate Names: Solute carrier family 18, member 3; solute carrier family 18 (vesicular monoamine) member 3; solute carrier family 18 (vesicular acetylcholine), member 3; rVAT; VACht, anti-VAT, anti-VACht

Gene Symbol: Slc18a3RRID: AB_572269Database Links:Entrez Gene:6572HumanEntrez Gene:20508MouseEntrez Gene: 287721 Rat