The ImmunoStar VMAT1 antiserum was quality control tested using standard immunohistochemical methods in rat adrenal medulla using biotin/avidin-HRP techniques; the antiserum shows no reactivity in rat CNS. Specificity of the antiserum was demonstrated by soluble pre-adsorption and Western blot. Tissue staining is completely eliminated by pretreatment of the diluted antibody with an excess of rat VMAT1 peptide residues 502-521. Western blot analysis of immunoprecipitated rat adrenal homogenates demonstrates a dense immunoreactive band of approximately 55 kD and a minor band of approximately 75 kD.

Photo Description: IHC image of VMAT1 staining in the rat adrenal medulla. The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for frozen sectioning. Sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.

Host:Rabbit

Quantity / Volume: 100 µL

State: Lyophilized Whole Serum

Species Reactivity: Rat

Availability: In Stock

Alternate Names: Chromaffin granule amine transporter; Solute carrier family 18 member 1; VAT1; VMAT-1; Vesicular amine transporter 1; Slc18a1. Cgat, Vmat1,Vmat-1; solute carrier family 18 (vesicular monoamine), member 1. Slc18a1,25693, anti-VMAT1

Gene Symbol: Slc18a1RRID: AB_10013883Database Links:Entrez Gene:25693Rat