Theantibodyisprovidedas100uLofaffinitypurifiedseruminPBS(0.02Msodiumphosphatewith0.15Msodiumchloride,pH7.5)with1%BSA(bovineserumalbumin),and0.02%sodiumazide. Reactswithmouse,rat,andrabbitspecies.

Theimagesabovelabeled"ImmunoStarRabbit5-HT2Areceptorantibody"aretheresultsofstainingofthemouse5-HT2Areceptorinthebrain,courtesyofDr.MagdalenaZaniewska,Max-Delbruck-CentrumfurMolekulareMedizinBerlin,Germany.

TheImmunoStar5HT2Areceptorantibodywasqualitycontroltestedusingstandardimmunohistochemicalmethods.Theantiserumdemonstratesstronglypositivelabelingofratcortex,amygdalaandhippocampususingindirectimmunofluorescentandbiotin/avidin-HRPtechniques.Recommendedprimarydilutionsare1/300-1/500inPBS/0.3%TritonX-100 -Bn/Av-HRPTechnique.Theadditionofintensifyingreagentssuchasnickelammoniumsulfatetothechromogensolutionwillapproximatelydoublethedilutionfactorasrecommended.

Immunolabelingiscompletelyabolishedbypreadsorptionwithsyntheticrat5HT2Areceptor(22-41).ImmunolabelingofWesternblotrevealedasinglebandofapproximately53kD.

PhotoDescription: LowmagnificationIHCimageofneuronsstainingforthe5-HT2Areceptorintheratcortex(topofpage)andimageofneuronalexpressionofthereceptorintheamygdala(below).Thebottomrightphotoisofthecortex. Thetissuewasfixedwith4%formaldehydein0.1Mphosphatebuffer,beforebeingremovedandpreparedforvibratomesectioning.FloatingsectionswereincubatedatRTin10%goatseruminPBS,beforestandardIHCprocedure.Primaryantibodywasincubatedat1:500for48hours,goatanti-rabbitsecondarywassubsequentlyaddedfor1hourafterwashingwithPBS.Lightmicroscopystainingwasachievedwithstandardbiotin-streptavidin/HRPprocedureandDABchromogen.

  
   

Host:Rabbit

Quantity/Volume:100uL

State:Liquid

ReactsWith:Human,Marine,Mouse,Rabbit,Rat

AlternateNames: 5-hydroxytryptaminereceptor2A;5Ht-2;5-hydroxytryptamine(SEROtonin)receptor2A,Gprotein-coupled,anti-5-HT2A

GeneSymbol/ID,Accession#: Htr2a,29595

RRID: AB_572211